|
KMID : 1094720130180050888
|
|
Biotechnology and Bioprocess Engineering
2013 Volume.18 No. 5 p.888 ~ p.896
|
|
|
Enhanced catalytic ability of Candida rugosa lipase immobilized on pore-enlarged hollow silica microspheres and cross-linked by modified dextran in both aqueous and non-aqueous phases
|
|
Ren Liwei
Jia Honghua
Yu Min
Shen Wenzhong
Zhou Hua
Wei Ping
|
|
|
Abstract
|
|
|
|
Candida rugosa lipase (CRL) is one of the most widely used lipases. To enhance the catalytic abilities of CRL in both aqueous and non-aqueous phases, hollow silica microspheres (HSMSs) with a pore size of 18.07 nm were used as an immobilization support, and aldehydecontaining dextrans were employed to further cross-link the adsorbed CRL. In the experimental ranges examined, the loading amount of lipase linearly increased to 171 ± 3.4 mgprotein/gsupport with the CRL concentration and all the adsorption equilibriums were reached within 30 min. After simple cross-linking, the tolerance to pH 4.0 ∼ 8.0 as well as the thermal stability of immobilized CRL at 40 ∼ 80°C were both substantially increased, and 82 ± 2.1% activity remaining after the sixth reuse. The immobilized CRL was successfully applied to the resolution of racemic ibuprofen in non-aqueous phase. The initial reaction rate increased by 1.4- and 3.6-fold compared with the rates of adsorbed and native lipases, respectively. Furthermore, the R-ibuprofen was obtained at ee > 93%, and the enantiomeric ratio reached E > 140 at the conversion of 50 ± 1.5% within 48 h.
|
|
|
KEYWORD
|
|
|
Candida rugosa lipase, hollow silica microsphere, dextran, immobilization, resolution
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|